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Liquid cultures of puri ed CD34+ (HPC) with a preestablished MSC feeder layer result in the expansion of CD34+/38+ HPC and CD34+/38 hematopoietic SCs and support the growth of mature hematopoietic total nucleated cell (TNC) progeny (Figure 9). Genetic Stability and Potential Tumorigenicity Genetic analysis of human MSCs is not well established. The signi cance of standard metaphase chromosome G banding is limited due to the low number of metaphases recovered during standard analyses. Advances in multicolor uorescence in situ hybridization (FISH) and highresolution array-based techniques may also soon be translated into practicable diagnostic tools in relation to CBMP safety in regenerative medicine [55]. Genetic instability can occur as a rare event after extended culture of mouse and human MSCs in FBS-supplemented medium [56, 57]. To test for potential in vivo tumor formation, MSCs derived from short-term clinical-scale expansions in FBSor HPL-supplemented media were injected into immunocompromised athymic nude mice subcutaneously. Putative tumor formation was evaluated by histological analyses three months after injection of 2 106 and 2 104 MSCs and compared to controls that were injected 48 h prior to euthanasia. A primary cell deposit was visible immediately and 48 h after injection and MSCs could be recovered by conventional microscopic evaluation. However, none of 12 animals tested developed a macroscopic or microscopic detectable tumor over the 90-day observation period [23]. In this situation, genetic testing may be encouraged for prospective data acqui-

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< xml version= 1.0 encoding= UTF-8 > <beans xmlns= http://www.springframework.org/schema/beans xmlns:xsi= http://www.w3.org/2001/XMLSchema-instance xsi:schemaLocation= http://www.springframework.org/schema/beans http://www.springframework.org/schema/beans/spring-beans-2.0.xsd > <bean id= screen class= com.wrox.begspring.ScreenWriter /> <bean id= add class= com.wrox.begspring.OpAdd /> <bean id= opsbean class= com.wrox.begspring.CalculateSpring autowire= byType /> </beans>

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The simple act of adding more access points so that coverage overlaps was covered in 9. The essential point to consider is that each access point on the same wireless network must have the same SSID value. The SSID value is the network name by definition. When you are overlapping wireless access points, they must use different channels (either 1, 6, or 11). Once you start adding wireless bridges these facts still apply. However, when you use repeaters in place of access points they use the same channels for transmission. If you have two separate networks in your home and office, each with its own separate address pools, and you wish to bridge them, you would normally connect two of the hubs or switches on each network with a wired connection. If a direct Ethernet connection isn t possible, you might try establishing a connection using the electrical wiring in your house or office. There are instances where even those kinds of connections aren t possible or desirable and it makes sense to bridge two networks with wireless devices. The essential connection sets up an access point on each LAN in a bridging mode and then connects the networks together. Figure 10-4 shows a D-Link access point being set for bridging mode.

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The Open Shortest Path First (OSPF) protocol is a dynamic routing protocol that uses the link state between nodes to determine routing paths for packets The link state is simply the state of the link to the next router (the neighbor) Routers in an OSPF environment do not check the distance from one point to another in a network Instead, the routers monitor the state of a link to each of its neighbor routers (the router next door) The link states are logged into the link state database (LSDB), which is then shared with all the neighbors LSDB information that is received is used to build the routing table for the router and then the information is shared with its neighbors Although an OSPF system can be a single autonomous system, most often OSPF routers are assigned as members of OSPF areas.

By the way, you can also initialize properties at the time that you declare them, much like you can with variables:

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