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This relates to the introduction of advanced but still user-friendly GC MS/MS instrument which are rapidly replacing GC MS for trace analysis in the pharmaceutical industry and elsewhere This chapter will thus focus on GC MS/MS methodology for detection of 3-OH FAs (markers for LPS) and Mur (a marker for PG), which are each well established [3 8] Ergosterol is also used as a marker for fungal contamination using GC MS/MS [9] Endotoxicity results from the interaction of a bacterial cell envelope component (eg, LPS or PG with a cell surface receptor constituting part of the nonspeci c immune system, (ie, a toll-like receptor on white blood cells) This results in the production of cytokines [eg, interleukin 1 (IL-1) or tumor necrosis factor (TNF)] as part of an intracellular enzyme cascade which can cause severe tissue injury.

q(w;t ) = -

Bioassays or immunoassays can be used to detect such reactions respectively As noted above the most widely used bioassay is the LAL assay A lysate of amoebocytes of the horseshoe crab (Limulus) contains an enzymatic clotting cascade which is activated by extremely low levels of LPS (nanogram levels or lower) There are variants of this assay that can detect PG, but they are not as widely used As noted above, other bioassays employ cultured cell lines that respond to LPS or PG, respectively Unfortunately bioassays are highly amenable to false positives (from the presence of cross-reactive substances) or false negatives from inhibition (by contaminants present in the sample) [10] A detailed discussion of these assays is beyond the scope of this chapter and has been reviewed elsewhere [1]..

When you select multiple cells, you can also merge the cells. Right-click and choose Merge Cells from the shortcut menu. By merging cells, you can create fairly complex table structures. You can also edit tables using grips. To understand editing tables with grips, imagine that the left side of the table is the stable side, while the right side of the table is the flexible side. The top-left grip is the base point for the entire table. You can do the following edits with grips: Upper-left grip: Moves the entire table. Upper-right grip: Stretches the table horizontally. As you change the width of the table, the columns also stretch proportionally. Lower-left grip: Stretches the table vertically. As you change the height of the table, the rows also stretch proportionally. Lower-right grip: Stretches the table both vertically and horizontally. The columns and rows adjust proportionally. Top-of-column grip: Adjusts the width of the column to the left of the grip. The entire table adjusts accordingly. If you press Ctrl while moving a column grip, the adjacent columns adjust, but the width of the table remains unchanged. You can export a table to comma-delimited (.csv) format. You can then open the table data with a database or spreadsheet program. To export a table, follow these steps:

37 The fact that CFCs are denser than air does not result in their settling to ground level and away from the stratosphere. Such settling though possible in undisturbed masses of air will be short lived, and air currents will readily carry the CFCs into the stratosphere.

Other effects such as captions are a personal preference. In general, you should leave the captions off since they do detract from the picture. They are helpful in identifying pictures at certain times so it is a great feature to have when you need it.